Neurological Disorders, Photoacoustic Imaging
Neurodegenerative disorders such as Alzheimer's disease (AD) are characterized by a decline in cognitive abilities that result from neuronal cell death. It is believed that hypoxia, oxidative stress, metal ion signaling, and neurotransmission all play a role in the neuropathology of AD; however, their interplay is inadequately studied, especially in the context of in vivo biological systems. Our group is currently developing chemical and protein-based probes to discover the mechanisms underlying this medical condition.
Photoacoustic (PA) imaging is an emerging modality that is based on the detection of acoustic waves generated by optically excited chromophores. Owing to the low scattering of sound in biological tissues, PA is ideal for non-invasive, deep-tissue bioimaging of live animals and human organs. Our group will develop novel approaches and chemical probes to detect biologically relevant analytes and molecular signaling events using PA imaging. These tools will be applied to explore cancer biology, diagnosis, and treatment.
Chan J, Tang A, Bennet AJ. Transition-state structure for the hydronium ion-promoted hydrolysis of ?-D-glucopyranosyl fluoride. Can J Chem 2014, Just-IN. article
Dodani SC,* Firl A,* Chan J,* Nam CI, Aron AT, Onak CS, Ramos-Torres KM, Paek J, Webster CM, Feller MB, Chang CJ. Copper is an endogenous modulator of neural circuit spontaneous activity. PNAS 2014, 111, 16280-1625. [* = equal contribution] article
Hong-Hermesdorf A, Miethke M, Gallaher SD, Kropat J, Dodani SC, Chan J, Barupala D, Domaille DW, Shirasaki DI, Loo JA, Weber PK, Pett-Ridge J, Stemmler TL, Chang CJ, Merchant SS. Subcellular metal imaging identifies dynamic sites of Cu accumulation in Chlamydomonas. Nature Chem. Biol. 2014, 10, 1034-1042. article
Chan J, Sannikova N, Tang A, Bennet J. Transition-state structure for the quintessential SN2 reaction of a carbohydrate: Reaction of ?-glucopyranosyl fluoride with azide ion in water. J. Am. Chem. Soc. 2014, 136, 12225-12228. article
Kashyap DR, Rompca A, Gaballa A, Helmann JD, Chan J, Chang CJ, Gupta D, Dziarski R. Peptidoglycan recognition proteins kill bacteria by inducing oxidative, thiol, and metal stress. PLoS Pathog. 2014, 10, 1-17. article
Polishchuk EV, Concilli M, Iacobacci S, Chesi G, Pastore N, Piccolo P, Paladino S, Baldantoni D, van IJzendoorn SCD, Chan J, Chang CJ, Amoresano A, Pane F, Pucci P, Tarallo A, Parenti G, Brunetti-pierri N, Settembre C, Ballabio A, Polishchuck RS. Wilson disease protein ATP7B utilizes lysosomal exocytosis to maintain copper homeostasis. Dev. Cell. 2014, 29, 686-700. article
Chan J, Chang CJ. Making light of stress. Nature Biotechnol. 2014, 32, 337-338. article
Huang CP, Fofana M, Chan J, Chang CJ, Howell SB. Copper transporter 2 regulates intracellular copper and sensitivity to cisplatin. Metallomics 2014, 6, 654-661. article
Hao Z, Lou H, Zhu R, Zhu J, Zhang D, Zhao BS, Zeng S, Chen X, Chan J, He C, Chen PR. The multiple antibiotic resistance regulator MarR is a copper sensor in Escherichia coli. Nature Chem. Biol. 2014, 10, 21-28. article
Au-Yeung HY, Chan J, Chantarojsiri T, Chang CJ. Molecular imaging of labile iron(II) pools in living cells with a turn-on fluorescent probe. J. Am. Chem. Soc. 2013, 135, 15165–15173. article
Chan J, Dodani SC, Chang CJ. Reaction-based small-molecule fluorescent probes for chemoselective bioimaging. Nature Chem. 2012, 2, 973–984. article