Effects and mechanisms of environmental factors on behavior and neurotransmission in rodent models
Research in the Eubig laboratory centers on determining the effects and mechanisms of environmental factors (circadian disruption; developmental or adult exposure to environmental contaminants and drugs of abuse) on behavior and neurotransmission in rodent models. We are also interested in the interaction of these factors with thyroid hormone. The emphasis of our lab is to use neuroscience techniques such as operant behavioral-testing methods, pharmacologic drug challenges, and molecular methods to address toxicology-related research questions. Current and planned projects include examining the effects of chronic circadian disruption on impulsive behavior and underlying cholinergic and dopaminergic signaling; examining the effects of organophosphorus (OP) insecticide exposure under the circadian disruption paradigm; and evaluating the effects of developmental methamphetamine exposure on attention and impulsivity. We use automated operant-conditioning chambers to assess the performance of contaminant-exposed rodents on tasks such as the 5-choice serial reaction time task, delay discounting, differential reinforcement of low rates of responding (DRL), and delayed spatial alternation. These tasks assess the cognitive domains of response inhibition (impulsive behavior), attention, and working memory, which are mediated by pre-frontal and striatal brain regions. In addition, we evaluate changes in synaptic protein expression (i.e., transporters, receptors) in response to our experimental manipulations. Other areas of interest to Paul Eubig include comparative neurotoxicology, clinical veterinary toxicology, communication of scientific information to the public, and ethics in science.
Project with Dr. Megan Mahoney (UIUC) evaluating the effects of chronic circadian disruption on impulsive behavior and underlying cholinergic and dopaminergic signaling.
Project with Drs. Chip Vorhees and Michael Williams (Cincinnati Children's Hospital Medical Center) evaluating the effects of developmental methamphetamine exposure on attention and impulsivity.
Project with Dr. Mary Gilbert (US EPA) evaluating the effects of subtle maternal hypothyroidism on cognitive functioning.
2016-2017 UIUC Research Board: Acetylcholine- and Dopamine-Related Pharmacologic Challenges to Investigate Impulsive Behavior due to Circadian Disruption in a Rodent Model
2009-2014 NIH NIEHS: Effects of PCBs and PBDEs on Three Distinct Components of Response Inhibition
Sable HJ, Eubig PA, Powers BE, Wang VC, Schantz SL (2009). Developmental exposure to PCBs and/or MeHg: effects on a differential reinforcement of low rates (DRL) operant task before and after amphetamine drug challenge. Neurotoxicol Teratol, 31(3):149-158. PMCID: PMC2730353.
Aguiar A, Eubig PA, Schantz SL (2010). Attention deficit/hyperactivity disorder: a focused overview for children’s environmental health researchers. Environ Health Perspect, 118(12):1646-1653. PMCID: PMC3002183.
Eubig PA, Aguiar A, Schantz SL (2010). Lead and PCBs as risk factors for attention deficit/hyperactivity disorder. Environ Health Perspect, 118(12):1654-1667. PMCID: PMC3002184.
Sable HJ, Monaikul S, Poon E, Eubig PA, Schantz SL (2011). Discriminative stimulus effects of cocaine and amphetamine in rats following developmental exposure to polychlorinated biphenyls (PCBs). Neurotoxicol Teratol, 33(2):255-262. PMCID: PMC3050088.
Merola VM, Eubig PA (2012). Toxicology of avermectins and milbemycins (macrocyclic lactones) and the role of P-glycoprotein in dogs and cats. Vet Clin North Am Small Anim Pract, 42(2):313-333. PMID: 22381182.
Eubig PA, Noe TE, Floresco SB, Sable JJ, Schantz SL (2014). Sex differences in response to amphetamine in adult Long-Evans rats performing a delay-discounting task. Pharmacol Biochem Behav, 118:1-9. PMID: 24388843.
Eubig PA, Westerink RHS. 2015. Co-guest editor for special issue: neurotoxicity of brominated flame retardants and the quest for safer alternatives. Neurotoxicol Teratol, 52(Pt B):118.
Bandara SB, Eubig PA, Sadowski RN, Schantz SL. 2016. Developmental PCB exposure increases audiogenic seizures and decreases glutamic acid decarboxylase in the inferior colliculus. Toxicol Sci, 149(2):335-345.