Role of Omega-3 and omega-6 fatty acids in Neurodegenerative Diseases
Discovery of novel anti-inflammatory lipid metabolites based on endocannabinoids and elucidation of their activities.
CURRENT ACTIVE COLLABORATIONS:
INTERNAL Prof. Timothy Fan, Veterinary Clinical Medicine, UIUC Prof. Logan Liu, Department of Electrical Engineering, UIUC Prof. Kris Kilian, Department of Material Science and Engineering, UIUC Prof. Rodney Johnson, Department of Nutritional Sciences, UIUC Prof. Andrew Smith, Department of Bioengineering, UIUC
EXTERNAL Prof. Michael Holinstat, Medical School Pharmacology, University of Michigan Prof. James Kincaid, Marquette University Dr. Ajikumar Parayil, Manus Biosynthesis, Boston.
2015. American Heart Association, National Scientist Development Grant 2013-Present: Industrial Funding from Manus Biosynthesis 2013- Research Board Grant
Bradley*, WB., Rouck*, J.E., Kambalyal, A., Das, A*. and Parayil, A*., "Orthogonal assays uncover enzyme promiscuity and chemical isomerization and remove ambiguity surrounding key P450 mediated oxygenation in Taxol biosynthesis". ACS-Chemical Biology, 2016, ASAP
Plucinski, L., Gartia, M., Arnold, W., Ameen, A., Chang, T., Hsiao, A., Liu, G.*, Das, A.*, "Substrate Binding to CYP2J2- Nanodiscs Detected by Nanoplasmonic Lygurcus Cup Arrays" Biosens Bioelectron., 2016, 337-46.
Lim, SJ, McDougle, D., Das, A* and Smith, A.*, "Lipoprotein Nanoplatelets: Fluorescent, Zwitterionic Probes for Molecular and Cellular Imaging." Journal of the American Chemical Society, 2015 Accepted with major revisions
Roy, J. Pondenis, H., Fan T.*, Das, A*, "Direct Capture of Functional Proteins from Mammalian Plasma Membranes into Nanodiscs" Biochemistry, 2015, 6299–6302.
McDougle, D*., Baylon, J.* Meling D., Kambalyal, A., Tajkorshid, E.*, and Das, A* "Incorporation of Charged Residues in the CYP2J2 F-G Loop Disrupts CYP2J2-Lipid Bilayer Interactions" Biochimica et Biophysica Acta -Biomembranes, 2015, 2060-2470.
Zelasko, S, Meling, D. and Das A*. "A critical residue that controls the isomerization of prostaglandin in thromboxane synthase", Biophysical Chemistry, 2015, 34-40.
Wilcox, K., Das, A., Velasco, P., Sligar S.G. and Klein, W. "Nanoscale synaptic membrane mimetic allows unbiased high throughput screen that targets binding sites for Alzheimer’s-associated A? oligomers" 2015, PLOS ONE In Press
Zelasko, S., Arnold, W., Das, A* " Endocannabinoid Metabolism by Cytochrome P450 Monooxygenases.", Prostaglandins and Other Lipid Mediators. 2015, http://www.sciencedirect.com/science/article/pii/S1098882314000744
McDougle, D., Kambalayal, A., Meling, D. and Das, A*. "Endocannabinoids - Anandamide and 2- Arachidonoylglycerol are Substrates for Human CYP2J2 Epoxygenase" Journal of Pharmacology and Experimental Therapeutics, 2014, 616-27.
Meling, D., McDougle, D. and Das, A.*,"CYP2J2 epoxygenase membrane anchor plays an important role in facilitating electron transfer from CPR." Journal of Inorganic Biochemistry 2014, 47-53.
Orlando, B., McDougle, D., Lucido, M., Eng, E., Stokes, D., Das, A*., and Malkowski, M*. "Cyclooxygenase-2 Catalysis and Inhibition in Lipid Bilayer Nanodiscs" Archives of Biochemistry and Biophysics 2014, 546, 33-40.
Srivarma, S., Mularcyzk, C. Meling, D. and Das, A*. "Substrate binding thermodynamics and kinetics to thromboxane synthase in model lipid bilayers", ChemBioChem. 2014, 15, 892
Zelasko S., Palaria, A., and Das, A* "Modifications to produce high-level expression of mammalian cytochrome P450s in recombinant expression systems" Protein Expression and Purification, 2013,92,77-87
McDougle, D*., Palaria, A.*Magnetta E., Meling, D. and Das, A*. "Functional Studies of N-terminally modified CYP2J2 epoxygenase in Model Lipid Bilayers", Protein Science, 2013, 22,964 (Amrita Palaria and Daniel McDougle are equal authors)