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Wang, Yingxiao (Peter)
Assistant Professor, Bioengineering, Beckman Institute for Advanced Science and Technology
B.S., Peking University, P.R. China
Ph.D., University of California, San Diego
Research Areas
Molecular Imaging and Cellular Engineering
My research interests include the development of genetically-encoded reporters based on fluorescent resonance energy transfer (FRET), and in combination with red fluorescence protein (RFP), to simultaneously visualize multiple signaling events and elucidate the molecular hierarchy involved in cellular signaling transduction. Another main research field of interest in the lab is to integrate the cutting-edge Nanotechnology and Optical Tools, e.g. Nano-fabrication, Laser-Tweezer, and Multiphoton Microscopy, together with fluorescence probes, to manipulate and visualize the regulatory signaling cascades in live cell motility, mechanobiology, and cancer cell development.
Representative Publications
Ouyang M., Sun J., Chien S., Wang Y. (2008) Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors, Proc Natl Acad Sci USA. 105(38):14353-8
Wang, Y., Shyy Y.-J., Chien, S. (2008) Fluorescence Proteins, Live Cell Imaging, and Mechanobiology: Seeing is Believing, Annual Review of Biomedical Engineering, 10:1-38
Lu S., Ouyang M., Seong J., Zhang J., Chien, S., Wang Y. (2008) The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging. PLoS Computational Biology, 4(7): e1000127, (Featured Research by PLoS Computational Biology)
Na S., Collin O., Chowdery F., Tay B., Ouyang M., Wang Y., Wang N. (2008) Rapid signal transduction in living cells is a unique feature of mechanotransduction. Proc Natl Acad Sci USA. 105 (18):6626-31
Ouyang M., Lu S., Li X.-Y., Xu J., Seong J., Giepmans B., Shyy Y.-J., Weiss SJ, Wang Y. (2008) Directional MT1-MMP Activity in Live Cells Visualized by a FRET Biosensor, Journal of Biological Chemistry. 2
83 (25):17740-8Wang Y., Lu S. (2008) The Application of FRET Biosensors to Visualize Src Activation. Proc. of SPIE. 6868 (68680A):1-9
Wang Y., Shyy, J.-Y., and Chien, S., (2008) “Fluorescence Live-Cell Imaging: Principles and Applications in Mechanobiology”. Bioengineering in Cell and Tissue Research, pp. 65-84
Wang Y. and Chien, S., (2007) “Analysis of Integrin Signaling by Fluorescence Resonance Energy Transfer”. Methods in Enzymology, 426:177-201
Botvinick, EL and Wang Y., (2007) “Laser Tweezers in the Study of Mechanobiology in Live Cells”. Methods in Cell Biology, 82:497-523
Wang Y., Chang JM., Chen K.-D., Li S., Li J., Wu C., Chien S. (2007) Selective Adapter Recruitment and Differential Signaling Networks by VEGF vs. Shear Stress. Proc Natl Acad Sci USA. 104 (21): 8875-9
Larina IV., Wang Y., Chien S., Lane ME., and Dickinson ME. (2007) On the way to subcellular imaging of mechanotransduction in the developing vasculature. Proc. of SPIE. 6535 (653504):1-6
Hu YL., Haga JH., Miao H., Wang Y., Li YS., Chien S. (2006) Roles of microfilaments and microtubules in paxillin dynamics. Biochem Biophys Res Commun. 348(4):1463-71.
Wang Y., Jin G., Miao H., Li J., Usami S., Chien S. (2006) Integrins Regulate VE-cadherin and Catenins: Dependence of This Regulation on Src, but not on Ras. Proc Natl Acad Sci USA. 103(6):1774-79.
Wang Y., Botvinick E., Zhao Y., Berns M., Usami S., Tsien R.Y., Chien S. (2005) " Visualization of Mechanical Activation of Src," Nature 434:1040-1045.
Miao H., Hu Y., Shiu Y.-T., Yuan S., Zhao Y., Kaunas R., Wang Y., Jin G., Usami S., and Chien S. (2005) "Effects of Flow Patterns on the Localization and Expression of VE-Cadherin at Vascular Endothelial Cell Junctions : In-vivo and In-vitro Investigations," Journal of Vascular Research 42:77-89.
Wang Y., Chang JM., Li Y.-C., Li Y.-S., Shyy J. Y.-J., and Chien S. (2004) "Shear Stress and VEGF Activate IKK via Flk-1/Cbl/Akt Signaling Pathway," Am J Physiol Heart Circ Physiol. 286:H685-692.
Shiu Y.-T., Li S., Yuan S., Wang Y., Nguyen P., Chien S. (2003) "Shear stress-induced c-fos activation is mediated by Rho in a calcium-dependent manner," Biochem Biophys Res Commun. 303:548-555.
Wang Y., Miao H., Li S., Chen K.-D., Li Y.-S., Yuan S., Shyy J. Y.-J., and Chien S. (2002) "Interplay between integrins and FLK-1 in shear stress- induced signaling," Am J Physiol Cell Physiol. 283:C1540-1548.
Additional Information
Awards:
Research Recognition Award, The American Physiological Society and International Union of Physiological Sciences, 2005
Caroline tum Suden/Frances Hellebrandt Professional Opportunity Award, The American Physiological Society, 2002
Graduate Student Research Award, Biomedical Engineering Society, 2001
Collaborative Projects:
Professor Rhanor Gillette - The roles of seritonin and NO regulating feeding behavior in several model organisms
Professor Klaude Weiss (Mt. Sinai School of Medicine, NY) - Discovering new neuropeptides and neurohormones in the simpler nervous system of Aplysia californica
Professor Leonid Moroz (University of Florida) - Single cell proteomics and analysis to uncover the roles of NO in cotransmission
Related Research (By Area):
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