Peptidomic characterization of rat dorsal root ganglion cells
I study the chemical content of rat dorsal root ganglion (DRG) neurons using analytical chemistry techniques such as mass spectrometry (MS) and microfluidics. The goal is to identify intercellular signaling molecules involved in the relay of sensory information from the periphery to the central nervous system. This work has the potential to uncover important therapeutic targets for a variety of sensory disorders, particularly those involving debilitating pain and itch. My most recent publication (see below) includes a characterization of the peptide content of DRG cells through the use of restricted space cultures and matrix assisted laser desorption/ionization (MALDI) MS.
Tillmaand EG*, Yang N*, Kindt CA*, Romanova EV, Rubakhin SS, Sweedler, JV. Peptidomics and secretomics of the mammalian peripheral sensory-motor system. J Am Soc Mass Spectrom. 2015 Dec;26(12):2051-2061. PMCID: PMC4655166.
Ong T-H, Tillmaand EG, Makurath M, Rubakhin SS, Sweedler JV. Mass spectrometry-based characterization of endogenous peptides and metabolites in small volume samples. Biochimica et Biophysica Acta. 2015; 1984(7):732-40. PMCID: PMC4426231.
Boerckel JD, Chandrasekharan UM, Waitkus MS, Tillmaand EG, Bartlett R, Dicorleto PE. Mitogen-activated protein kinase phosphatase-1 promotes neovascularization and angiogenic gene expression. Arterioscler Thromb Vasc Biol. 2014 May;34(5):1020-31. PMCID: PMC4121075
Bandyopadhyay S, Harris DP, Adams GN, Lause GE, McHugh A, Tillmaand EG, Money A, Willard B, Fox PL, Dicorleto PE. HOXA9 methylation by PRMT5 is essential for endothelial cell expression of leukocyte adhesion molecules. Mol Cell Biol. 2012 Apr;32(7):1202-13. PMCID: PMC3302442
*These authors contributed equally to the work